Abstract

ABSTRACT - The aim of the study was to compare the effects of slow freezing and vitrification on blastomere/chromatin integrity and energy/oxidative stress parameters of mouse preimplantation embryos. Mouse (4-cell to blastocyst stage) embryos were analyzed as fresh (controls) or after vitrification or slow freezing. Embryo collapsing was found after both slow freezing (P<0.001) and vitrification (P<0.05). Significantly higher rate of blastomere cytofragmentation was found after slow freezing (P<0.001) but it was not observed after vitrification. Chromatin damage and altered mitochondrial (mt) distribution pattern were observed, both after slow freezing (P<0.05) and vitrification (P<0.05). In embryos at the morula stage, mt activity was reduced by slow freezing (P<0.05) but it did not change by vitrification. In embryos at the blastocyst stage, mt activity was reduced by both slow freezing (P<0.05) and vitrification (P<0.05). Intracellular reactive oxygen species (ROS) level was significantly lower in slow-frozen (P<0.05) but higher in vitrified embryos (P<0.05) compared with controls. Mitochondria/ROS colocalization was significantly reduced after slow freezing (P<0.05) but it was not affected by vitrification. In conclusion, this study demonstrates that vitrification is a suitable method to preserve embryo bioenergy/redox parameters.

Autore Pugliese

• Dell'aquila Maria Elena

Tutti gli autori

• DELL'AQUILA M.E.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2012

ISSN

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ISBN

978-88-907328-0-5

Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

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Settori ERC

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Codici ASJC

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