Characterization of differentially expressed transcripts in quercetin-treated apples by suppression subtractive hybridization

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Abstract

In previous trials the flavonoid quercetin proved to be effective in reducing Penicillium expansum infections and patulin accumulation in apples. Since quercetin resulted more effective in in vivo than in in vitro trials, a possible role of this substance in enhancing host resistance was hypothesized. To verify this hypothesis, a cDNA library of genes differentially expressed in response to quercetin application was constructed by using the suppression subtractive hybridization (SSH) approach. A total of 89 unique sequences were obtained. By homology search and functional analysis the identified sequences were putatively categorized as belonging to “metabolism”, “subcellular localization” and “protein with binding functions or cofactor requirement” classes. Similarity was also found with genes coding proteins whose role in defence mechanisms is still unknown.

Autore Pugliese

Tutti gli autori

  • IPPOLITO A.;NIGRO F.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2010

ISSN

0567-7572

ISBN

978-90-66056-13-8

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

1

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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