Assembly and characterization of pandemic influenza A H1N1 genome in nasopharyngeal swabs using high-throughput pyrosequencing

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Abstract

De novo high-throughput pyrosequencing was used to detect and characterize 2009 pandemic influenza A (H1N1) virus directly in nasophatyngeal swabs in the context of the microbial community Data were generated with a prior sequence-independent amplification by 454 pyrosequencing on GS-FLX platform (Roche). Influenza A assembled reads allowed near full-length genome reconstruction with the simultaneous analysis of site-specific heterogeneity. The molecular approach applied proved to be a powerful tool to characterize the new pandemic H1N1 influenza virus in clinical samples. This approach could be of great value in identifying possibly new reassortants that may occur in the near future.

Autore Pugliese

Tutti gli autori

  • PICARDI E.;PESOLE G.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2011

ISSN

1121-7138

ISBN

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Numero di citazioni Wos

Nessuna citazione

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Numero di citazioni Scopus

11

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Settori ERC

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Codici ASJC

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