Agreement between the cell culture titrations of canine minute virus determined by two susceptibility-testing methods

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Abstract

The correct diagnosis of canine minute virus is critical in dog breeding. In this study, the Bland Altman test was used to compare the performance of two susceptibility-testing methods, namely polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA). The agreement between IFA and PCR in monocytes revealed a mean difference of -1752.16 with 95% confidence and an interval ranging from -3229.80 to -274.53 (SD=2325.62). The agreement between IFA and PCR in Walter Reed canine cells (WRCC) revealed a mean difference of -2396.55 with 95% confidence and an interval ranging from -3774.63 to -1018.48 (SD=2168.93). The Bland Altman test confirmed the overall accuracy of PCR vs IFA and the plot showed that all points were not randomly arranged in the range of average ±1.96×SD of the differences.

Autore Pugliese

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  • PRATELLI A.;COLAO V.

Titolo volume/Rivista

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Anno di pubblicazione

2013

ISSN

1532-2971

ISBN

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