Abstract

Aim: Notwithstanding the widely accepted idea that following disuse skeletal muscles become faster, an increase in shortening velocity was previously observed mostly in fibers containing type 1 myosin, whereas a decrease was generally found in fibers containing type 2B myosin. In this study unloaded shortening velocity of pure type 1 and 2B fibers from hindlimb unloaded mice was determined and a decrease in type 2B fibers was found. Methods: To clarify whether the decrease in shortening velocity could depend on alterations of myosin motor function, an in vitro motility assay approach was applied to study pure type 1 and pure type 2B myosin from hindlimb unloaded mice. The latter approach, assessing actin sliding velocity on isolated myosin in the absence of other myofibrillar proteins, enabled to directly investigate myosin motor function. Results: Actin sliding velocity was significantly lower on type 2B myosin following unloading (2.70±0.32 μms-1) than in control conditions (4.11±0.35 μms-1), whereas actin sliding velocity of type 1 myosin was not different following unloading (0.89±0.04 μms-1) compared to control conditions (0.84±0.17 μms-1). Myosin light chain isoform composition of type 2B myosin from hindlimb unloaded and control mice was not different. No oxidation of either type 1 or 2B myosin was observed. Higher phosphorylation of regulatory myosin light chain in type 2B myosin after unloading was found. Conclusion: Results suggest that the observed lower shortening velocity of type 2B fibers following unloading could be related to slowing of actomyosin kinetics in the presence of myosin light chain phosphorylation.

Autore Pugliese

• Desaphy Jean Francois

Tutti gli autori

• CONTE D.;DESAPHY J.F.

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2014

ISSN

1748-1708

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

6

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile