Abstract

The probiotic potential of Lactobacillus plantarum and Lactobacillus fermentum strains, capable of overproducing riboflavin, was investigated. The riboflavin production was quantified in co-cultures of lactobacilli and human intestinal epithelial cells, and the riboflavin overproduction ability was confirmed. When milk and yogurt were used as carrier matrices, L. plantarum and L. fermentum strains displayed a significant ability to survive through simulated gastrointestinal transit. Adhesion was studied on both biotic and abiotic surfaces. Both strains adhered strongly on Caco-2 cells, negatively influenced the adhesion of Escherichia coli O157:H7, and strongly inhibited the growth of three reference pathogenic microbial strains. Resistance to major antibiotics and potential hemolytic activity were assayed. Overall, this study reveals that these Lactobacillus stains are endowed with promising probiotic properties and thus are candidates for the development of novel functional food which would be both enriched in riboflavin and induce additional health benefits, including a potential in situ riboflavin production, once the microorganisms colonize the host intestine.

Autore Pugliese

• Spano Giuseppe

Tutti gli autori

• M.P. Arena , P. Russo , V. Capozzi , P. López , D. Fiocco , G. Spano

Titolo volume/Rivista

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Anno di pubblicazione

2014

ISSN

0175-7598

ISBN

Non Disponibile

Numero di citazioni Wos

26

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

28

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile