Abstract

Aims: Psychosocial stress alters the HPA-axis. Increasing evidence shows a link between these alterations and oxidant elevation. Oxidative stress is implicated in the stress-response and in the pathogenesis of neurologic and psychiatric diseases. NOX enzymes are a major source of ROS in the CNS. Here, we investigated the contributory role of NOX2-derived ROS to the development of neuroendocrine alterations in a rat model of chronic psychosocial stress, the social isolation. Results: Significant elevations in the hypothalamic levels of CRF and plasmatic ACTH were observed from four weeks of social isolation. Increased levels of peripheral markers of the HPA-axis (plasmatic and salivary corticosterone) were observed at later time point of social isolation (seven weeks). Alteration in exploratory activity of isolated rats followed the same time course. Increased expression of markers of oxidative stress (8OhdG and nitrotyrosine) and NOX2 mRNA was early detectable in the hypothalamus of isolated rats (after two weeks), but later (after seven weeks) in the adrenal gland. A three-weeks-treatment with the antioxidant/NOX inhibitor apocynin stopped the progression of isolation-induced alterations of the HPA-axis. Rats with a loss of function mutation in the NOX2 subunit p47phox were totally protected from alterations of the neuroendocrine profile, behavior and increased NOX2 mRNA expression induced by social isolation. Innovation: We demonstrate that psychosocial stress induces early elevation of NOX2-derived oxidative stress in the hypothalamus and consequent alterations of the HPA-axis, leading ultimately to altered behavior. Conclusion: Pharmacological targeting of NOX2 might be of crucial importance for treatment of psychosocial stress-induced psychosis.

Autore Pugliese

• Tucci Paolo

Tutti gli autori

• Colaianna, Marilena , Schiavone, Stefania , Zotti, Margherita , Tucci, Paolo , Morgese, Maria Grazia , Baeckdahl, Liselotte , Holmdahl, Rikard , Krause, Karl-Heinz , Cuomo, Vincenzo , Trabace, Luigia

Titolo volume/Rivista

ANTIOXIDANTS & REDOX SIGNALING

Anno di pubblicazione

2013

ISSN

1523-0864

ISBN

Non Disponibile

Numero di citazioni Wos

39

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

40

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile