Abstract

Poly(ADP-ribose) polymerase (PARP) is a 116kDa enzyme catalysing the synthesis of ADP-ribose polymers from NAD+. PARP is activated in response to DNA strand breaks and plays a critical role in the maintenance of genomic integrity. However, considering its role also in transcription, proliferation as well as apoptosis in biological process, in the present study the role of PARP in bone regeneration was evaluated, in particular in bone cell proliferation and differentiation processes. Thus, formalin fixed paraffin embedded specimens of 10 human bone samples after sinus lift were collected and investigated by immunohistochemistry using a mouse monoclonal anti-human PARP antibody. PARP was expressed in cells with morphological features of osteoblasts in the areas of new bone formation at the junction between mineralized and unmineralized tissue, between osteoid tissue and bone. Few osteoclasts were observed and showed only focal nuclear expression of PARP, while osteocytes showed no positivity for PARP. Our data showed an overall involvement of PARP enzyme in human bone tissues, in particular during bone regeneration process.

Autore Pugliese

• Lo Russo Lucio

Tutti gli autori

• LO MUZIO L. , Pannone G. , Santarelli A. , LO RUSSO L. , DE LILLO A. , Rubini C. , Bambini F. , Bufo P. , Dioguardi M. , Procaccini M.

Titolo volume/Rivista

JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS

Anno di pubblicazione

2014

ISSN

0393-974X

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

Non Disponibile

0

Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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