Determination of biogenic amines in wine by means of thin-layer chromatography/densitometry

Abstract

Biogenic amines are small-molecular-weight organic bases that can be encountered in all fermented foods, including wine. Ingestion of wine containing biogenic amines, and especially histamine, can result in health nuisances. HPLC is the analytical technique most often employed in the determination of biogenic amines in wine but HPLC-based methods are expensive and time-consuming. A new method, based upon amine dansylation and TLC/densitometry, was developed and validated. This allowed for the determination of histamine, tyramine, putrescine and cadaverine in wine at concentrations between 1 and 20 mg/L. Analytical performances adequately complied with the needs of routine wine analysis, moreover the method was high-throughput and inexpensive. A simpler, semi quantitative version of the method, based on visual evaluation of spot intensity, was also developed.


Autore Pugliese

Tutti gli autori

  • A. Romano , H. Klebanowski , S. La Guerche , L. Beneduce , G. Spano , M.L. Murat , P. Lucas

Titolo volume/Rivista

FOOD CHEMISTRY


Anno di pubblicazione

2012

ISSN

0308-8146

ISBN

Non Disponibile


Numero di citazioni Wos

34

Ultimo Aggiornamento Citazioni

Non Disponibile


Numero di citazioni Scopus

36

Ultimo Aggiornamento Citazioni

Non Disponibile


Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile