Abstract

Matrix Assisted Pulsed Laser Evaporation (MAPLE) was used as an alternative technique within strategies for enzyme immobilization to produce Laccase thin films. In order to characterize Laccase thin films, suitable deposition substrates were used. Laccase layers deposited onto silicon were investigated by Fourier Transform InfraRed Spectroscopy (FTIR) and Atomic Force Microscopy (AFM) to study molecular structure and surface morphology of Laccase thin films, respectively. Moreover, to estimate the amount of deposited enzyme, Quartz Cristal Microbalance (QCM) electrodes modified with Laccase films were used. Finally, glass and screen printed carbon electrodes were used as deposition substrates to test by colorimetric assay and amperometry the activity of the Laccase deposited. Experimental results have demonstrated that MAPLE enables the Laccase deposition that retains 10% of the initial activity.

Autore Pugliese

• Centonze Diego

Tutti gli autori

• Cicco N. , Morone A. , Verrastro M. F. , Dinescu M. C. , Matei A. , Mitu B. , Centonze D.

Titolo volume/Rivista

LECTURE NOTES IN ELECTRICAL ENGINEERING

Anno di pubblicazione

2015

ISSN

1876-1100

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

2

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile