Abstract

Dental pulp stem cells (DPSCs) are an adult stem cells population with high proliferative potential and the ability to differentiate in many cell types, and this has led scientists to consider these cells to be an alternative source of postnatal stem cells comparable to mesenchymal stem cells from bone marrow. In this work, we studied the osteoblastic phenotype developed by DPSCs cultured in osteogenic medium. In particular, we analyzed the expression of the typical osteoblast markers such as alkaline phosphatase, collagen type I, osteocalcin, osteopontin, as well as mineralized matrix production. Furthermore, the gene expression during DPSC differentiation into osteoblastic cells was studied by microarray technology. Using microarray and reverse transcriptase–polymerase chain reaction analysis, we found that IGFBP-5, JunB, and NURR1 genes are upregulated during the differentiation of DPSCs. These data indicate that opportunely differentiated DPSCs show a correct osteoblastic phenotype. Therefore, during the osteoblastic differentiation process, genes such as IGFBP-5, JunB, and NURR1 significantly increase their expression.

Autore Pugliese

• Mori Giorgio

Tutti gli autori

• G. Mori , G. Brunetti , A. Oranger , C. Carbone , A. Ballini , L. Lo Muzio , S. Colucci , C. Mori , F.R. Grassi , M. Grano.

Titolo volume/Rivista

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES

Anno di pubblicazione

2011

ISSN

0077-8923

ISBN

Non Disponibile

Numero di citazioni Wos

39

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

39

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile