Abstract

Raman spectroscopy is a powerful technique for studying cellular biochemistry. In fact, each toxic chemical induces biochemical changes related to the own action mechanism. In this investigation Raman microspectroscopy has been used, in correlation with atomic force microscopy images, to detect biochemical and structural damages occurring in culturedhumancells as a consequence of deltamethrin exposure. Cultured human keratinocyte cells have been exposed at increasing concentrations of deltamethrin from 10−3M to 10−6M for 24 h. A viability test indicated that the cytotoxic dose corresponds to exposure at deltamethrin solution for 24 h with the chemical concentration between 10−4Mand 2.5 10−4 M. The compared analysis of Raman spectra and AFM images allows to state that an evident damage occurs in the plasmaticmembraneand it is already detectable after exposure of keratinocytes at the lowest investigated deltamethrin concentration (10−6 M). The most important modifications are related to the breakdown of CH2 bonds of lipidic chains, whereas proteineous bonds are less involved in the deltamethrin action. On the whole, cellular damage starts after exposure to deltamethrin doses well lower than that established as cytotoxic. © 2011 Elsevier B.V. All rights reserved

Autore Pugliese

• Perna Giuseppe

Tutti gli autori

• G. Perna , M. Lasalvia , P. D'Antonio , G. Quartucci , V. Capozzi

Titolo volume/Rivista

VIBRATIONAL SPECTROSCOPY

Anno di pubblicazione

2011

ISSN

0924-2031

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

6

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile