Biotechnological Production of Vitamin B2-Enriched Bread and Pasta
Abstract
Lactic Acid Bacteria (LAB) were obtained from durum wheat flour samples and screened for roseoflavin-resistant variants in order to isolate natural riboflavin-overproducing strains. Two riboflavin-overproducing strains of Lactobacillus plantarum isolated as described above were used for the preparation of bread (by means of sourdough fermentation) and pasta (using a pre fermentation step) in order to enhance their vitamin B2 content. Pasta was produced from a monovarietal semolina obtained from the durum wheat cultivar PR22D89 and from a commercial re-milled semolina. Several samples were collected during the pasta-making process (dough, extruded, dried and and cooked pasta) and tested for their riboflavine content by a high performance liquid chromatography method. The applied approaches resulted in a considerable increase of vitamin B2 content (about a two-fold and three-fold increase in pasta and bread, respectively), thus representing a convenient and efficient food-grade biotechnological application for the production of vitamin B2-enriched bread and pasta. This methodology may be extended to a wide range of cereal-based foods, feed and beverages. Additionally, our work exemplifies the production of a functional food by a novel biotechnological exploitation of LAB in pasta-making.
Autore Pugliese
Tutti gli autori
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Capozzi V. , Menga V. , Digesù A.M. , De Vita P. , van Sinderen D. , Cattivelli L. , Fares C. , Spano G.
Titolo volume/Rivista
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Anno di pubblicazione
2011
ISSN
0021-8561
ISBN
Non Disponibile
Numero di citazioni Wos
4
Ultimo Aggiornamento Citazioni
Non Disponibile
Numero di citazioni Scopus
38
Ultimo Aggiornamento Citazioni
Non Disponibile
Settori ERC
Non Disponibile
Codici ASJC
Non Disponibile
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