Abstract

A Laccase-based biosensor for the determination of phenolic compounds was developed by using Matrix Assisted Pulsed Laser Evaporation as an innovative enzyme immobilization technique. and the deriving biosensor was characterized and applied for the first time. Laccase was immobilized onto different substrates including screen printed carbon electrodes and spectroscopic, morphologic and electrochemical characterizations were carried out. A linear range from 1 to 60 M was achieved working at 5.5 pH and -0.2 V detection potential vs Ag pseudoreference. The limits of detection and quantification were found to be 1 and 5 M, respectively. A good fabrication reproducibility, stability of response and selectivity toward interferents were also found The potential of the developed biosensor was tested in the determination of total polyphenol content in real matrices (tea infusion, ethanolic extract from Muscari comosum bulbs and aqueous solution of a food supplement from black radish root and artichoke leaves) and the results were compared with those obtained by using the Folin-Ciocalteu method.

Autore Pugliese

• Centonze Diego

Tutti gli autori

• Verrastro M. , Cicco N. , Crispo F. , Morone A. , Dinescu M. , Dumitru M. , Favati F. , Centonze D.

Titolo volume/Rivista

TALANTA

Anno di pubblicazione

2016

ISSN

0039-9140

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

6

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile