Abstract

AbstractBackground Selective imaging of lysosomes by fluorescence microscopy using specific fluorescent probes allows the study of biological processes and it is potentially useful also for diagnosis. Lysosomes are involved in numerous physiological processes, such as bone and tissue remodeling, plasma membrane repair, and cholesterol homeostasis, along with cell death and cell signaling. Despite the great number of dyes available today on the market, the search for new fluorescent dyes easily up-taken by cells, biocompatible and bearing bright and long-lasting fluorescence is still a priority. Methods Two thiophene-based fluorescent dyes, {TC1} and TC2, were synthetized as lysosome-specific probes. Results The new dyes showed high selectivity for fluorescent staining and imaging of lysosomes and disclosed high photostability, low toxicity and pH insensitivity in the range 2–10. Conclusions The {TC} dyes exhibited high co-localization coefficients (> 95%) and moderate quantum yields. They showed high biocompatibility and long-term retention, important features for biological applications. General significance The results of the present work disclose a new class of organic dyes with potential wide applications as specific and efficient lysosome probes in the study of various biological processes.

Autore Pugliese

• Papadia Paride , Ciccarella Giuseppe , Gigli Giuseppe , Vergaro Viviana

Tutti gli autori

• A. L. Capodilupo , V. Vergaro , F. Baldassarre , A. Cardone , G. A. Corrente , C. Carlucci , S. Leporatti , P. Papadia , G. Gigli , G. Ciccarella

Titolo volume/Rivista

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Anno di pubblicazione

2015

ISSN

0304-4165

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

6

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile