Abstract

Semiquinone oscillations induced by light pulses in the presence of exogenous electron donors are a valuable source of information on the kinetics and thermodynamics of ubiquinone chemistry relevant to the QB site of the photosynthetic reaction center (RC). In previous attempts to achieve the quantitative interpretation of data, the ubiquinone concentration was considered constant during the experiment since it was much bigger than that of RC. In this work, we extended existing models to low ubiquinone concentrations revealing several hidden processes taking place during the ubiquinone photoreduction and enabling the evaluation of the ubiquinone binding constant KQ at the QB site. The proposed approach provides for the first time the evaluation of KQ without any preliminary treatment of ubiquinone extraction from the binding site, thereby better preserving its native structure.

Autore Pugliese

• Giotta Livia

Tutti gli autori

• Ciriaco, F. , Tangorra, R. , Antonucci, A. , Giotta, L. , Agostiano, A. , Trotta, M. , Milano, F.

Titolo volume/Rivista

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Anno di pubblicazione

2015

ISSN

0175-7571

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

3

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile