Abstract

The purpose of this study was to evaluate ‘‘in field’’ the accumulation of virus in shellfish and compare it with the concentration of bacterial indicators. Individuals of Mytilus galloprovincialis were placed in two sampling station located in a contaminated coastal bay and in one control station located one kilometer offshore. The presence of Rotavirus and E. coli was assessed weekly both in seawater and in shellfish samples. The Rotavirus genome was detected in water, preliminarily concentrated by tangential flow ultrafiltration method, and in hepatopancreas of mussels by Real-Time PCR. E. coli was enumerated in water matrices by a filtering method and in mussels by the MPN method. Rotaviruses were not recorded in seawater, while in mussels they were detected since third week after placement. E. coli in mussels were always below the limits set in the Regulation (EC) 854/2004. This study suggests the need for a viral indicator to insure the safety for consumption of shellfish.

Autore Pugliese

• De Donno Maria Antonella

Tutti gli autori

• Bagordo F. , Grassi T. , Idolo A. , Serio F. , Gabutti G. , De Donno A.

Titolo volume/Rivista

FOOD AND ENVIRONMENTAL VIROLOGY

Anno di pubblicazione

2013

ISSN

1867-0334

ISBN

Non Disponibile

Numero di citazioni Wos

3

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

6

Ultimo Aggiornamento Citazioni

22/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile