Abstract

High conductance Ca2+-activated K+ channels (BK channels) have previously been demonstrated in the eel intestine. They are specifically activated following a hypotonic stress and sustain Regulatory Volume Decrease (RVD). The aim of the present work was to address the possible role of these channels in the Apoptotic Volume Decrease (AVD) of isolated eel enterocytes, and the possible interaction between BK channels and the progression of apoptosis. The detection of apoptosis was performed by confocal microscopy and annexin V and propidium iodide labelling; cell volume changes were monitored by video imaging. Within a few hours after isolation, enterocytes underwent anoikis (apoptosis induced by detachment from the extracellular matrix). They showed an early normotonic volume decrease (AVD) preceding the appearance of annexin V positivity. AVD occurred in correspondence with an increase in the [Ca2+]i, measured with Fura-2. When the cells were resuspended in high K+ solution or treated with iberiotoxin, AVD was completely abolished. In addition, treatment with high K+ or iberiotoxin significantly inhibited apoptosis progression. It was demonstrated for the first time in native enterocytes that BK channels, which are involved in RVD in these cells, plays also a crucial role in the AVD process and in the progression of apoptosis

Autore Pugliese

• Lionetto Maria Giulia

Tutti gli autori

• Lionetto M.G. , Giordano M.E. , Calisi A. , Caricato R. , Hoffmann E.K. , Schettino T

Titolo volume/Rivista

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY

Anno di pubblicazione

2010

ISSN

1015-8987

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

6

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile