Quartz crystal microbalance with dissipation (QCM-D) as tool to exploit antigen–antibody interactions in pancreatic ductal adenocarcinomadetection

Torna indietro

Abstract

Novel syntheticpeptidesrepresentsmartmoleculesforantigen–antibodyinteractionsinseveral bioanalyticsapplications,frompurificationtoserumscreening.Theirimmobilizationontoasolid phase isconsideredakeypointforsensitivityincreasing.Inthisview,weexploitedQuartzCrystal Microbalancewithsimultaneousfrequencyanddissipationmonitoring(QCM-D)withadoubleaim, specifically,asinvestigativetoolforspacersmonolayerassemblinganditsfunctionalevaluation,as well ashighsensitivemethodforspecificimmunosorbentassays.Themethodwasappliedto pancreaticductaladenocarcinoma(PDAC)detectionbystudyingtheinteractionsbetweensynthetic phosphorylatedandun-phosphorylated a-enolasepeptideswithseraofhealthyandPDACpatients.The syntheticpeptideswereimmobilizedonthegoldsurfaceoftheQCM-Dsensorviaaself-assembled alkanethiolmonolayer.Thepresentedexperimentalresultscanbeappliedtothedevelopmentof surfaceslesssensitivetonon-specificinteractionswiththefinaltargettosuggestspecificprotocolsfor detectingPDACmarkerswithun-labeledbiosensors.

Autore Pugliese

Tutti gli autori

  • M. Bianco , A. Aloisi , V. Arima , M. Capello , S. Ferri-Borgogno , F. Novelli , S. Leporatti , R. Rinaldi

Titolo volume/Rivista

BIOSENSORS & BIOELECTRONICS

Anno di pubblicazione

2013

ISSN

0956-5663

ISBN

Non Disponibile

Numero di citazioni Wos

15

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

16

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile