Abstract

Several bioengineering approaches have been proposed for peripheral nervous system repair, with limited results and still open questions about the underlying molecular mechanisms. We assessed the biological processes that occur after the implantation of collagen scaffold with a peculiar porous microstructure of the wall in a rat sciatic nerve transection model compared to commercial collagen conduits and nerve crush injury using functional, histological and genome wide analyses. We demonstrated that within 60 days, our conduit had been completely substituted by a normal nerve. Gene expression analysis documented a precise sequential regulation of known genes involved in angiogenesis, Schwann cells/axons interactions and myelination, together with a selective modulation of key biological pathways for nerve morphogenesis induced by porous matrices. These data suggest that the scaffold's microstructure profoundly influences cell behaviors and creates an instructive micro-environment to enhance nerve morphogenesis that can be exploited to improve recovery and understand the molecular differences between repair and regeneration.

Autore Pugliese

• Sannino Alessandro , Madaghiele Marta , Salvatore Luca

Tutti gli autori

• Cerri F. , Salvatore L. , Memon D. , Martinelli Boneschi F. , Madaghiele M. , Brambilla P. , Del Carro U. , Taveggia C. , Riva N. , Trimarco A. , Lopez I.D. , Comi G. , Pluchino S. , Martino G. , Sannino A. , Quattrini A.

Titolo volume/Rivista

BIOMATERIALS

Anno di pubblicazione

2014

ISSN

0142-9612

ISBN

Non Disponibile

Numero di citazioni Wos

18

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

17

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile