Abstract

We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.

Autore Pugliese

• Piro Gabriella , Di Sansebastiano Gian Pietro

Tutti gli autori

• Di Sansebastiano G.P. , Fornaciari S. , Barozzi F. , Piro G. , Arru L.

Titolo volume/Rivista

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Anno di pubblicazione

2014

ISSN

1422-0067

ISBN

Non Disponibile

Numero di citazioni Wos

11

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

14

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile