Abstract

Optical stimulation and silencing of neural activity is a powerful technique for elucidating the structure and function of neural circuitry. In most invivo optogenetic experiments, light is delivered into the brain through a single optical fiber. However, this approach limits illumination to a fixed volume of the brain. Here a focused ion beam is used to pattern multiple light windows on a tapered optical fiber. We show that such fibers allow selective and dynamic illumination of different brain regions along the taper. Site selection is achieved by a simple coupling strategy at the fiber input, and the use of asingle tapered waveguide minimizes the implant invasiveness. We demonstrate the effectiveness of this approach for multipoint optical stimulation in the mammalian brain invivo by coupling the fiber to a microelectrode array and performing simultaneous extracellular recording and stimulation at multiple sites in the mouse striatum and cerebral cortex.

Autore Pugliese

• De Vittorio Massimo

Tutti gli autori

• F. Pisanello , L. Sileo , I.A. Oldenburg , M. Pisanello , L. Martiradonna , J.A. Assad , B.L. Sabatini , M. De Vittorio

Titolo volume/Rivista

NEURON

Anno di pubblicazione

2014

ISSN

0896-6273

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

58

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile