Abstract

In this chapter, we discuss several immunohistochemical methods to monitor apoptosis and autophagy in two models, the cultured rat neuroblastoma cells and the developing rat cerebellum . Apoptosis and autophagy have been implicated in many physiological and pathological processes. Apoptotic cell death is characterized by membrane blebbing and nuclear chromatin condensation /fragmentation , alteration of cytoskeleton components and cytoplasmic organelles , and formation of membrane-enveloped apoptotic bodies that are rapidly phagocytosed by macrophages . Autophagy is a sequential set of events including double membrane formation and elongation, vesicle maturation , and finally delivery of the targeted materials to the lysosome . Platinum compounds can induce different apoptotic pathways in neural cell cultures and alter the normal balance between the formation and degradation of cellular proteins . These compounds also affect cell death by apoptosis and autophagy in the proliferating and differentiating cells of rat cerebellum in vivo .

Autore Pugliese

• Fanizzi Francesco Paolo

Tutti gli autori

• M. Bottone , F. Fanizzi , G. Bernocchi

Titolo volume/Rivista

Non Disponibile

Anno di pubblicazione

2015

ISSN

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ISBN

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Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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