Abstract

The development of agents that can modulate microglial activation has been suggested as one potential strategy for the treatment or prevention of neurodegenerative diseases. Among these agents, resveratrol, with its anti-inflammatory action, has been described to have neuroprotective effects. In this paper we demonstrate that in LPS-stimulated microglia resveratrol pretreatment reduced, in a dose-dependent manner, pro-inflammatory cytokines IL-1β, TNF-α and IL-6 mRNA expression and increased the release of anti-inflammatory interleukin (IL)-10. Moreover, resveratrol pretreatment up-regulated the phosphorylated forms of JAK1 and STAT3, as well as suppressor of cytokine signaling (SOCS)3 protein expression in LPS activated cells, demonstrating that the JAK-STAT signaling pathway is involved in the anti-inflammatory effect exerted by resveratrol. By supplementing the cultures with an IL-10 neutralizing antibody (IL-10NA) we obtained the opposite effect. Taken together, these data allow us to conclude that the LPS-induced pro-inflammatory response in microglial cells can be markedly reduced by resveratrol, through IL-10 dependent up-regulation of SOCS3, requiring the JAK-STAT signaling pathway.

Autore Pugliese

• Lofrumento Dario Domenico

Tutti gli autori

• Cianciulli A. , Dragone T. , Calvello R. , Porro C. , Trotta T. , Lofrumento D.D. , Panaro M.A.

Titolo volume/Rivista

INTERNATIONAL IMMUNOPHARMACOLOGY

Anno di pubblicazione

2015

ISSN

1567-5769

ISBN

Non Disponibile

Numero di citazioni Wos

27

Ultimo Aggiornamento Citazioni

25/04/2018

Numero di citazioni Scopus

30

Ultimo Aggiornamento Citazioni

26/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile