Abstract

The development of bio-devices for complete regeneration of ligament and tendon tissues is presently one of the biggest challenges in tissue engineering. Such device must simultaneously possess optimal mechanical performance, suitable porous structure, and biocompatible microenvironment. This study proposes a novel collagen-BDDGE-elastin (CBE)-based device for tendon tissue engineering, by the combination of two different modules: (i) a load-bearing, non-porous, "core scaffold" developed by braiding CBE membranes fabricated via an evaporative process and (ii) a hollow, highly porous, "shell scaffold" obtained by uniaxial freezing followed by freeze-drying of CBE suspension, designed to function as a physical guide and reservoir of cells to promote the regenerative process. Both core and shell materials demonstrated good cytocompatibility in vitro, and notably, the porous shell architecture directed cell alignment and population within the sample. Finally, a prototype of the core module was implanted in a rat tendon lesion model, and histological analysis demonstrated its safety, biocompatibility, and ability to induce tendon regeneration. Overall, our results indicate that such device may have the potential to support and induce in situ tendon regeneration.

Autore Pugliese

• Sannino Alessandro , Salvatore Luca

Tutti gli autori

• Sandri M. , Filardo G. , Kon E. , Panseri S. , Montesi M. , Iafisco M. , Savini E. , Sprio S. , Cunha C. , Giavaresi G. , Veronesi F. , Fini M. , Salvatore L. , Sannino A. , Marcacci M. , Tampieri A.

Titolo volume/Rivista

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

Anno di pubblicazione

2016

ISSN

2296-4185

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

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Numero di citazioni Scopus

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Ultimo Aggiornamento Citazioni

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Settori ERC

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Codici ASJC

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