Abstract

A highly selective water compatible molecularly imprinted polymer (MIP) for 3-nitro-L-tyrosine (3NT), an oxidative stress marker associated with neurodegenerative disorders, was prepared and its use as solid-phase extraction (SPE) sorbent material was demonstrated. The MIP was prepared by bulk polymerization using methacrylic acid as functional monomer and acetonitrile as porogen with traces of acetic acid and trifluoroacetic acid. In order to evaluate its binding properties, the MIP was analyzed by batch rebinding experiments and subsequently used as SPE sorbent for the selective clean-up and pre-concentration of 3NT from standard solutions and spiked human urine samples. The results obtained from batch rebinding experiments showed the presence of two association constants corresponding to high-affinity (Ka 4.20X103 M-1) and low-affinity (Ka 0.79X103 M-1) binding sites. Standard mixture solution loaded on MIP-SPE cartridge gave a recovery of 95% for 3NT, while the other compounds were totally eluted during washing step. Percentage of recovery higher than 90%, with relative standard deviation of 2%, was also obtained when a maximum of 55 microg of 3NT is used in spiked urine sample and loaded into the cartridge. Validation of the analytical method for 3NT quantification in human urine gave 0.7 micromg mL-1 of limit of detection, a linear range of 2.5–55 microg mL-1 with a relative standard deviation of 2%.

Autore Pugliese

• Del Sole Roberta

Tutti gli autori

• L. Mergola , S. Scorrano , R. Del Sole , M.R. Lazzoi , G. Vasapollo

Titolo volume/Rivista

BIOSENSORS & BIOELECTRONICS

Anno di pubblicazione

2013

ISSN

0956-5663

ISBN

Non Disponibile

Numero di citazioni Wos

24

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

23

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile