Abstract

Very few studies have provided information about the effects of cadmium (Cd) at histoanatomical and ultrastructural levels, along with potential localization of the metal in planta. In particular, from this standpoint, almost nothing is known in Daucus carota L (carrot), a particularly important species for in vitro and in vivo functional investigations. In this work we hypothesized that 36 mu M Cd, supplied for 1, 2, 3, 4, 7 and 14 days to 30-day-old in vitro-cultured plants, might induce an early acclimation, but a final collapse of roots and leaves. In fact, as a general feature, a biphasic root response to Cd stress actually took place: in the first phase (1-4 days of Cd exposure), the cytological and functional events observed by light microscopy, TEM, epifluorescence, as well as by the time-course of thiol-peptide compounds can be interpreted as acclimatory responses aimed at diminishing the movement of Cd across the root. The second phase (from 4 to 14 days of Cd exposure) was instead characterized by cell hypertrophy, cell-to-cell separation events, increase in alpha-beta-gamma-tocopherol levels and, not least, endocytogenic processes, coupled with a dramatic drop in the amount of thiol-peptide compounds. These events led to a progressive root collapse, even if they did not ingenerate macro/microscopic injury symptoms in leaf blades and petioles. (C) 2012 Elsevier Masson SAS. All rights reserved.

Autore Pugliese

• Lenucci Marcello Salvatore

Tutti gli autori

• Sanità di Toppi L. , Vurro E. , De Benedictis M. , Falasca G. , Zanella L. , Musetti R. , Lenucci M.S. , Dalessandro G. , Altamura M.M.

Titolo volume/Rivista

PLANT PHYSIOLOGY AND BIOCHEMISTRY

Anno di pubblicazione

2012

ISSN

0981-9428

ISBN

Non Disponibile

Numero di citazioni Wos

Nessuna citazione

Ultimo Aggiornamento Citazioni

Non Disponibile

Numero di citazioni Scopus

Non Disponibile

Ultimo Aggiornamento Citazioni

Non Disponibile

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile