Abstract

Besides triiodothyronine (T3), 3,5-diiodo-l-thyronine (T2) has been reported to affect mitochondrial bioenergetic parameters. T2 effects have been considered as independent of protein synthesis. Here, we investigated the effect of in vivo chronic T2 administration to hypothyroid rats on liver mitochondrial FoF1-ATP synthase activity and expression. T2 increased state 4 and state 3 oxygen consumption and raised ATP synthesis and hydrolysis, which were reduced in hypothyroid rats. Immunoblotting analysis showed that T2 up-regulated the expression of several subunits (α, β, FoI-PVP and OSCP) of the ATP synthase. The observed increase of β-subunit mRNA accumulation suggested a T2-mediated nuclear effect. Then, the molecular basis underlying T2 effects was investigated. Our results support the notion that the β-subunit of ATP synthase is indirectly regulated by T2 through, at least in part, the activation of the transcription factor GA-binding protein/nuclear respiratory factor-2. These findings provide new insights into the T2 role on bioenergetic mechanisms.

Autore Pugliese

• Siculella Luisa , Damiano Fabrizio

Tutti gli autori

• Mangiullo R. , Gnoni A. , Damiano F. , Siculella L. , Zanotti F. , Papa S. , Gnoni G.V.

Titolo volume/Rivista

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Anno di pubblicazione

2010

ISSN

0005-2728

ISBN

Non Disponibile

Numero di citazioni Wos

25

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

26

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile