Abstract

Short-term effects of 3,5-L-diiodothyronine (T(2)) administration to hypothyroid rats on F(o)F(1)-ATP synthase activity were investigated in liver mitochondria. One hour after T(2) injection, state 4 and state 3 respiration rates were noticeably stimulated in mitochondria subsequently isolated. F(o)F(1)-ATP synthase activity, which was reduced in mitochondria from hypothyroid rats as compared to mitochondria from euthyroid rats, was significantly increased by T(2) administration in both the ATP-synthesis and hydrolysis direction. No change in β-subunit mRNA accumulation and protein amount of the α-β subunit of F(o)F(1)-ATP synthase was found, ruling out a T(2) genomic effect. In T(2)-treated rats, changes in the composition of mitochondrial phospholipids were observed, cardiolipin (CL) showing the greatest alteration. In mitochondria isolated from hypothyroid rats the decrease in the amount of CL was accompanied by an increase in the level of peroxidised CL. T(2) administration to hypothyroid rats enhanced the level of CL and decreased the amount of peroxidised CL in subsequently isolated mitochondria, tending to restore the CL value to the euthyroid level. Minor T(2)-induced changes in mitochondrial fatty acid composition were detected. Overall, the enhanced F(o)F(1)-ATP synthase activity observed following injection of T(2) to hypothyroid rats may be ascribed, at least in part, to an increased level of mitochondrial CL associated with decreased peroxidation of CL.

Autore Pugliese

• Siculella Luisa

Tutti gli autori

• Cavallo A. , Gnoni A. , Conte E. , Siculella L. , Zanotti F. , Papa S. , Gnoni G.V.

Titolo volume/Rivista

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES

Anno di pubblicazione

2011

ISSN

1573-6881

ISBN

Non Disponibile

Numero di citazioni Wos

15

Ultimo Aggiornamento Citazioni

28/04/2018

Numero di citazioni Scopus

16

Ultimo Aggiornamento Citazioni

28/04/2018

Settori ERC

Non Disponibile

Codici ASJC

Non Disponibile