METHOD FOR THE COLORIMETRIC DETECTION OF CONTAMINATION WITH NUCLEASES

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Abstract

Method for detecting the presence of nucleases in a sample, characterized in that it comprises the steps of: - incubating the sample to be tested for the presence of nucleases with at least one oligonucleotide linker constituting the substrate for the nuclease to be detected, for a sufficient time to cause degradation of said oligonucleotide linker by the nuclease possibly present in the sample, - adding to the sample, upon incubation, colloidal gold nanoparticles comprising gold nanoparticles functionalized with a first probe oligonucleotide and gold nanoparticles functionalized with a respective second probe oligonucleotide, said first and second probe oligonucleotides being complementary to a respective portion of the nucleotide sequence of the oligonucleotide linker, and - examining the possible colour change of the sample as a result of the addition of said nanoparticles, a colour change of the sample to the colour assumed by the colloidal gold particles when aggregated at a distance less than their size being indicative of the absence of the tested nuclease from the sample.

Classe Tecnologica

C - Chemistry and Metallurgy

Patent Office

European Patent Office

Numero Deposito

EP 3234176

Anno Deposito

2015

Anno Concessione

2017

Inventori Pugliesi

  • Pompa Pier Paolo
  • Cecere Paola

Tutti gli inventori

  • Pompa Pier Paolo
  • Valentini Paola
  • Cecere Paola

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  • Non ci sono titolari pugliesi

Tutti i titolari

  • Fondazione Istituto Italiano Di Tecnologia